Persona:
Pérez Esteban, Javier

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ORCID
0000-0003-0996-6795
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Pérez Esteban
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Javier
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2024 - 20252
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Nivel de acceso: info:eu-repo/semantics/openAccess × Fecha de inicio: 2020 ×

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Mostrando 1 - 2 de 2
  • Miniatura
    Publicación
    Use of magnetite nanoparticles and magnetic separation for the removal of metal(loid)s from contaminated mine soils
    (ELSEVIER, 2025) Caballero Mejia, Bibiana; Moliner, Ana; Hontoria Fernández, Chiquinquirá; Mariscal Sancho, Ignacio; Pérez Esteban, Javier; Escolástico León, Consuelo; https://orcid.org/0000-0002-0090-0741; https://orcid.org/0000-0001-5963-1964; https://orcid.org/0000-0002-8089-0327; https://orcid.org/0000-0003-2344-6710
    Magnetite nanoparticles have been successfully used for removal and immobilization of contaminants in water, yet their application in soils combined with in situ magnetic separation remains unexplored. We evaluated the effectiveness and optimal conditions for using magnetite nanoparticles combined with magnetic separation to remove metal(loid)s from contaminated mine soils. Soil samples were incubated (15, 45 days) with varying doses of magnetite (0, 25, 50 g kg⁻¹) and moisture (dry, field capacity) and separated using electromagnet or permanent magnet. This technique achieved up to 44 % As, 65 % Cd, 60 % Cu, 47 % Fe, 40 % Mn, 65 % Pb, and 62 % Zn removal, leaving minimal residual magnetite in the soil. These high removal efficiencies were attributed to the nanoparticles' magnetic properties, adsorption capacity and ability to form aggregates with soil particles. Optimal conditions were 25 g kg⁻¹ of magnetite incubated for 45 days at field capacity and separated by the electromagnet. Higher doses (50 g kg⁻¹) offered minimal improvement at increased costs. The combined use of magnetite nanoparticles and in situ magnetic separation demonstrated a low-impact and cost-effective method for reducing metal(loid) concentrations to levels that facilitate subsequent soil remediation strategies.
  • Miniatura
    Publicación
    Bioremediation of petroleum hydrocarbons polluted soil by spent mushroom substrates: Microbiological structure and functionality
    (Elsevier, 2024-05-21) Mayans, Begoña; Antón Herrera, Rafael; García Delgado, Carlos; Delgado Moreno, Laura; Guirado, María; Escolástico León, Consuelo; Eymar, Enrique; Pérez Esteban, Javier
    Spent mushroom substrate (SMS) holds valuable microbiota that can be useful in remediating polluted soils with hydrocarbons. However, the microorganisms behind the bioremediation process remain uncertain. In this work, a bioremediation assay of total petroleum hydrocarbons (TPHs) polluted soil by SMS application was performed to elucidate the microorganisms and consortia involved in biodegradation by a metabarcoding analysis. Untreated polluted soil was compared to seven bioremediation treatments by adding SMS of Agaricus bisporus, Pleurotus eryngii, Pleurotus ostreatus, and combinations. Soil microbial activity, TPH biodegradation, taxonomic classification, and predictive functional analysis were evaluated in the microbiopiles at 60 days. Different metagenomics approaches were performed to understand the impact of each SMS on native soil microbiota and TPHs biodegradation. All SMSs enhanced the degradation of aliphatic and aromatic hydrocarbons, being A. bisporus the most effective, promoting an efficient consortium constituted by the bacterial families Alcanivoraceae, Alcaligenaceae, and Dietziaceae along with the fungal genera Scedosporium and Aspergillus. The predictive 16 S rRNA gene study partially explained the decontamination efficacy by observing changes in the taxonomic structure of bacteria and fungi, and changes in the potential profiles of estimated degradative genes across the different treatments. This work provides new insights into TPHs bioremediation.